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raw deep-sequencing data  (Biotechnology Information)

 
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    Biotechnology Information raw deep-sequencing data
    Raw Deep Sequencing Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deep-sequencing+raw+data/deep+sequencing+data/pm37890669-454-0-11
    Average 90 stars, based on 1 article reviews
    raw deep-sequencing data - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: TMEM241 is a UDP-N-acetylglucosamine transporter required for M6P modification of NPC2 and cholesterol transport.
    Article Snippet: Raw deep-sequencing data have been deposited in the National Center for Biotechnology Information Sequence Read Archive (PRJNA1015588).

    Article Title: Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro−transfection
    Article Snippet: The raw data from targeted deep sequencing were submitted to the National Center for Biotechnology Information (NCBI) Bioproject ( http://www.ncbi.nlm.nih.gov/bioproject/ ) under the accession number PRJNA983990.

    Sequencing:

    Article Title: Integrated multi-omics analysis of metabolome and transcriptome profiles during bovine adipocyte differentiation reveals functional divergence of FADS2 isoforms in lipid metabolism regulation
    Article Snippet: .. Raw deep-sequencing data have been deposited in the National Center for Biotechnology Information Sequence Read Archive PRJNA1043825 ( https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1043825/ ) and PRJNA892230 ( https://www.ncbi.nlm.nih.gov/bioproject/PRJNA892230/ ). ..



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    Journal: Cell reports

    Article Title: Viral Fitness Landscapes in Diverse Host Species Reveal Multiple Evolutionary Lines for the NS1 Gene of Influenza A Viruses

    doi: 10.1016/j.celrep.2019.11.070

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Illumina deep-sequencing raw data , This manuscript , https://data.mendeley.com/datasets/77x6pyfk79/1 https://data.mendeley.com/datasets/ryr859j6pr/1.

    Techniques: Virus, Recombinant, Purification, One Step RT-PCR, Reverse Transcription, Gel Extraction, Library Quantification, DNA Library Preparation, Sample Prep, Software

    Approach for combined transcriptomics and viral sequencing of single influenza virus-infected cells that express IFN. (A) IFN reporter A549 cells are infected with a mix of wild-type and synonymously barcoded viruses. IFN + cells are enriched by MACS and pooled with nonenriched cells and uninfected canine cells that serve as controls for multiplets and mRNA leakage. (B) The mRNAs from individual cells are converted to cDNAs tagged with cell-specific barcodes. (C) Cellular transcriptomes are quantified using standard single-cell 3′-end Illumina sequencing, and (D) viral genes are enriched by influenza virus-specific PCR and fully sequenced by PacBio (in this schematic, only the cell labeled by the red barcode is infected and has viral transcripts that are sequenced by PacBio). (E) The result is a matrix giving the expression of each gene in each cell, as well as the full sequences of the viral genes in infected cells.

    Journal: Journal of Virology

    Article Title: Single-Cell Virus Sequencing of Influenza Infections That Trigger Innate Immunity

    doi: 10.1128/JVI.00500-19

    Figure Lengend Snippet: Approach for combined transcriptomics and viral sequencing of single influenza virus-infected cells that express IFN. (A) IFN reporter A549 cells are infected with a mix of wild-type and synonymously barcoded viruses. IFN + cells are enriched by MACS and pooled with nonenriched cells and uninfected canine cells that serve as controls for multiplets and mRNA leakage. (B) The mRNAs from individual cells are converted to cDNAs tagged with cell-specific barcodes. (C) Cellular transcriptomes are quantified using standard single-cell 3′-end Illumina sequencing, and (D) viral genes are enriched by influenza virus-specific PCR and fully sequenced by PacBio (in this schematic, only the cell labeled by the red barcode is infected and has viral transcripts that are sequenced by PacBio). (E) The result is a matrix giving the expression of each gene in each cell, as well as the full sequences of the viral genes in infected cells.

    Article Snippet: Briefly, the raw deep-sequencing data from the Illumina 3′-end sequencing were processed using the 10x Genomics software package cellranger (version 2.2.0).

    Techniques: Sequencing, Infection, Labeling, Expressing